蚕蛹中人生长激素类似脂蛋白的纯化方法

Method for purifying human growth hormone-like protein in silkworm pupa

Abstract

The invention belongs to a method for purifying human growth hormone-like protein in silkworm pupa. The method comprises the following steps of: pretreating the silkworm pupa; primarily separating the human growth hormone-like protein; performing affinity chromatography purification and ultrafiltration and concentration by using a product of a No.4 protein absorption peak, and the like. The silkworm pupa is used as a raw material, and is separated and purified to form natural medicinal protein for reducing blood viscosity and improving blood microcirculation; the method is easy to implement, the yield is high, and the method is suitable for large-scale production; and the purified natural protein can serve as a healthcare medicine without toxic or side effect.
本发明属于一种蚕蛹中人生长激素类似脂蛋白的纯化方法,方法包括蚕蛹预处理,人生长激素类似脂蛋白的初步分离,用4号蛋白吸收峰的所得品进行亲和层析纯化和超滤浓缩等步骤。本发明能利用蚕蛹作为原料,通过分离纯化获得具有降低血液粘稠度,改善血液微循环的天然药用蛋白,该方法操作简便,且产量高,适用于大规模生产,提纯的天然蛋白可作为保健药物,无毒副作用。

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    Publication numberPublication dateAssigneeTitle
    WO-2014075443-A1May 22, 2014天津耀宇生物技术有限公司Procédé de purification d'un groupe de protéines de 30 kd présent dans les pupes de ver à soie